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throat culture

What is a throat culture

Throat culture refers to a test that is often used to detect infections from a specimen taken from your throat. A throat culture or throat swab culture involves collecting a sample from the site of infection in your throat and inoculating the sample into solid or liquid nutrient media (e.g., agar, gelatin) in order to grow any microorganisms like bacteria or fungi that may be present. The throat culture test can help find the cause of a sore throat. Often, a sore throat is caused by a virus, but a throat culture will see if it’s definitely caused by strep bacteria, helping doctors decide on the proper treatment. The throat swab culture test results guide appropriate therapy in infectious and inflammatory diseases of the throat.

However, throat cultures can be very difficult to interpret. These specimens are not easily collected without introducing contamination from adjacent areas. Furthermore, the contaminating organisms from these types of specimens are often considered pathogenic. At times, evaluation of the specimen by examining the direct Gram stain can help determine the quality of the specimen and therefore the potential significance of the isolates.

Culture media include blood agar, chocolate agar and Mueller-Hinton tellurite blood agar or Tinsdale agar (if Corynebacterium diphtheriae infection is suspected). Incubation on BA is better for streptococcal hemolysis. If chocolate agar is used, it may be kept for 18-48 hours at 35-37°C.

What does a throat culture test for?

The most common bacterial cause of a sore throat is an infection with group A streptococcus also known Streptococcus pyogenes or group A beta-hemolytic streptococcus. If these harmful bacteria have been identified in the throat culture, then the affected person will be treated with antibiotics. Because the appropriate antibiotic therapy is predictable for group A streptococcal infection, no further testing is usually necessary.

However, other types of cultures that are positive for pathogenic bacteria may require an antibiotic susceptibility test, which grows the bacteria in the presence of a panel of antibiotics to see which antibiotics may be effective for treating the infection. For one example of a susceptibility method, the bacteria are swabbed onto an agar plate and antibiotics that are impregnated into filter paper disks are placed on the plate. Following an overnight incubation step, the absence of bacterial growth around the disk indicates the ability of that antibiotic to inhibit the bacteria. The results of this test inform the health practitioner which antibiotic to prescribe to cure the infection.

How long does a throat culture take?

The culture of the throat sample usually remains in the incubator for 18 to 24 hours to allow sufficient time for any bacteria that may be present to grow. Throat culture test results are generally ready in 2 days.

After incubation, a laboratory professional will visually examine the culture. Some bacteria have a characteristic appearance that enables the laboratory professional to presumptively identify the specific bacteria, but additional biochemical or serological tests are usually needed to make the identification. The laboratory professional must distinguish potential pathogenic bacteria from normal throat flora that are not harmful and don’t require treatment.

Throat swab culture test results

  • A normal or negative result means no bacteria or other germs that may cause a sore throat were found.
  • An abnormal or positive result means bacteria or other germs that can cause a sore throat were seen on the throat swab.

Strep throat culture

The bacteria Streptococcus pyogenes, also known as group A beta-hemolytic streptococcus or group A streptococcus, causes “strep throat,” the most common bacterial cause of inflammation and soreness of the back of the throat (pharyngitis). Strep throat tests identify the presence of these bacteria as the cause of a sore throat.

Strep tests are used to determine whether a person with a sore throat (pharyngitis) has strep throat, an infection of the throat and tonsils caused by the bacteria Streptococcus pyogenes, also called group A streptococcus.

Most sore throats are caused by a virus and will resolve without treatment within a few days, but some people with sore throats have strep throat. It is important to diagnose and treat strep infections promptly with antibiotics because they are very contagious and secondary complications can develop, especially in children.

A rapid strep test, also known as a rapid antigen detection test (RADT), can detect group A strep antigens. Results are available in 10-20 minutes. Recently, the U.S. Food and Drug Administration (FDA) approved molecular tests that can detect genetic material from group A strep bacteria in throat swab specimens in less than 8 minutes.

If the results of the rapid test are positive, further testing is not needed and treatment can be started right away. A positive rapid strep test indicates the presence of group A streptococci, the bacteria that cause strep throat. A negative rapid test indicates that the affected person probably does not have strep throat, but a throat culture may be performed for confirmation, especially among children and adolescents.

If the rapid strep test is negative, a throat culture should be performed on children or adolescents to confirm the results and avoid missing infections that could lead to serious complications, such as rheumatic fever. A throat culture is more sensitive than the rapid strep test, but it may take 24-48 hours for results. According to 2012 guidelines from the Infectious Diseases Society of America, confirmatory testing on adults is not usually recommended since adults have lower rates of strep throat and far lower risk of complications than children.

If the throat culture is positive for group A streptococci, then the person tested does have strep throat. In rare cases, pharyngitis may be caused by group C or group G streptococci. These organisms will not cause a positive rapid test but can be detected by culture. If the culture is negative, then it is most likely that the sore throat is due to a viral infection that will resolve on its own.

When is strep throat culture ordered?

Your doctor will typically order this test when a person has a sore throat and other symptoms that suggest strep throat. There is a higher suspicion of strep when the affected person is a child and/or if the person has been in close contact with someone who has been diagnosed with strep throat. Individuals should see a doctor and get tested when they have:

  • Sore throat that starts quickly and lasts more than a week, or recurrent sore throats
  • Fever of 101° F or higher
  • Reddened (inflamed) throat and/or tonsils with white or yellow patches or streaks
  • Tiny red spots at the back of the roof of the mouth
  • Difficulty or severe pain when swallowing
  • Headache, body aches
  • Nausea or vomiting
  • Tender and/or swollen throat or lymph nodes
  • Rash
  • Hoarseness lasting more than two weeks
  • Blood in saliva or mucus
  • Excessive drooling in young children
  • Symptoms of dehydration, such as excessive thirst, dry mouth, decreased urination

In accordance with the 2012 Infectious Diseases Society of America (IDSA) guidelines, testing is not recommended when there are also symptoms more closely associated with a viral infection, such as:

  • Cough
  • Runny nose
  • Mouth sores

Since strep is less common in children under the age of 3, Infectious Diseases Society of America advises against strep throat testing in this age group unless the child is considered at risk, such as when a family member has been diagnosed with strep throat. In general, the Infectious Diseases Society of America advises against the routine screening of asymptomatic people who have been in close contact with someone with a strep throat infection.

Note that the 2012 Infectious Diseases Society of America guidelines are voluntary and do not represent universal consensus in the medical community on when and how to use the strep test. They reflect current knowledge and expert opinion and are intended to assist in the diagnosis and management of strep throat.

Strep throat infection

Strep throat spreads from person-to-person through contact with respiratory secretions that contain the streptococcal bacteria. During influenza season, the early symptoms of influenza, such as fever, chills, headache, sore throat, and muscle pain, may mimic strep throat. To differentiate between strep and influenza, a rapid strep test and a rapid influenza test may be done at the same time.

Most people with streptococcal pharyngitis would eventually recover without antibiotic treatment, but they will be contagious for a longer period of time and are at a greater risk of developing secondary complications.

Strep throat is most common in 5 to 15 year olds. Some school children may be “carriers,” persons who have the bacteria but who have no symptoms. Carriers can still spread the infection to others.

Recent antibiotic therapy or gargling with some mouthwashes may affect the rapid strep test results.

Are there other types of streptococci that can cause a sore throat?

Group C and group G streptococci, normally found in animals, can occasionally cause pharyngitis in humans. However, these bacteria do not pose a risk for the serious secondary complications associated with group A streptococci. Antibiotic treatment for group A streptococci will be effective against these organisms as well.

What can I do to prevent the spread of strep throat?

The spread of strep throat can be reduced by good hand washing, especially after coughing and sneezing and before preparing food or eating. People with sore throats should be seen by a healthcare provider who can perform tests to find out whether the illness is strep throat. If the test result shows strep throat, the person should stay home from work, school, or daycare until 24 hours after taking an antibiotic.

How long does treatment for strep throat usually last?

Ten to 14 days, depending on the antibiotic prescribed. Although your symptoms may improve or disappear before you have taken all of your antibiotics, you should complete your full course of treatment by taking all of the pills that were prescribed.

How long should I stay away from other people if I have a positive test result?

You should complete at least 24 hours of antibiotics before close contact with others.

If one child in my family has strep throat, is everyone going to get sick?

Other family members, including adults, can be infected by the bacteria. Your healthcare provider may test all family members who have sore throats and may test children under the age of 3. In most cases, it is not necessary to test other family members who do not have symptoms.

When can my child go back to school?

Usually after one full day of therapy and absence of significant fever; however, a few small studies have found that children may return to school as soon as 12 hours after taking their first dose of antibiotic provided they no longer have a fever and their symptoms have improved.

I’ve had strep throat before and was treated with antibiotics. Can I get it again?

Yes. Although antibodies may protect those who have had previous strep infections, there are so many different strains of this organism that being immune to all of them is unlikely. Therefore, someone could potentially get strep throat again and again. The best way to decrease the risk of transmission to others is to minimize close contact with others when ill and wash hands often and thoroughly with soap and water or alcohol-based hand scrub.

Do other group A streptococous infections occur?

Group A streptococcus can also cause infections that occur separately from strep throat, such as impetigo and, rarely, more invasive conditions such as toxic shock syndrome or necrotizing fasciitis (the so-called “flesh-eating bacteria”).

What is an ASO test and how is it used to detect a strep infection?

Antistreptolysin O (ASO) is a blood test used to help diagnose a current or past infection with group A strep (Streptococcus pyogenes). Antistreptolysin O (ASO) blood test detects antibodies to streptolysin O, one of the many strep antigens. This test is rarely ordered now compared to thirty years ago. For an acute strep throat infection, the ASO test is not helpful; the rapid strep test or throat culture should be used. However, if a health practitioner is trying to find out if someone had a recent strep infection that may not have been diagnosed, this test could be helpful. In addition, it may be used to help diagnose rheumatic fever or glomerulonephritis, which occurs weeks after a strep throat infection when the rapid strep and throat culture would no longer be positive.

Health Jade Team

The author Health Jade Team

Health Jade